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Integration of Texas Isolate Reticuloendotheliosis Virus in Fowlpox Virus Isolated from a Chicken Pox Lesion
JeanMarie Stanley, Ellen W. Collisson1

1Department of Veterinary Pathobiology, Texas A&M University,
College Station, TX 77843-4467

Abstract Jean Marie pic

Objective: The purpose of this study is to determine if a Texas isolate of REV is integrated into the genome of the FPV of the associated pox lesion.  This study also hopes to show how the use of nested PCR for identification of positive birds has drastically reduced the number of positive birds in the APC captive breeding facilities.

Animals/Samples Used: A frozen pox lesion obtained during the necropsy of a domestic chicken in Texas; blood samples from Attwater’s prairie chickens (APC) in various captive breeding facilities across Texas

Procedure: After isolating the DNA from the pox lesion, the pox lesion DNA and control sample DNA was digested and re-ligated. This product was amplified by inverse PCR and the DNA separated using gel electrophoresis, and sequenced.  APC white blood cells were removed from the serum, the DNA was then extracted, a nested PCR was performed, and separated using gel electrophoresis.

Results: Sequencing of the bands of the pox lesion DNA and the positive control did not yield viable results.  The nested PCR from captive bred APCs show no positive birds.

Conclusions:  Under the conditions of this study, positive results for REV integration into the genome of FPV were not obtained.  Further research into this matter is imperative for the survival of the species. It is evident, that nested PCR has helped to dramatically reduce the number of positive APCs in the past year.  Without reducing the mortality due to REV, reintroduction efforts cannot keep up with the current population decline.