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Effects of Prozac on Neuron Division and Cell Death in the Hippocampus of Leaner Mice

Dana Tomlinson

Objective- To see if Prozac increases neuronal cell division and/or stops neuronal cell death in the hippocampus of leaner mice.

Animals- C57BL/6J wild type and leaner mice

Procedures-Mice were given intraperitoneal injections of Prozac with steril Lacteated Ringers as vehicle or vehicle only, for two weeks starting on postanatal day 10.  Postnatla day 23 and 24 they received injections of BrdU every eight hours and once on postnatal day 25.  The mice were then euthanized, perfused, and then their brains extracted for further study.  The brains were sectioned on a cryostate at 25 μm, and then stained using an immunohistochemistry technique that allowed for visualization of BrdU stained cells.

Preliminary Results-The hippocampus of both wild type and leaner mice treated with Prozac, appeared to have an increase in neuronal cell division.  The mice that received Lactated Ringers solution, however, had far fewer dividing cells.

Conclusions and Clinical Relevance-Since Prozac appears to increase neuronal cell division in the hipoocampus, it might beneficial for patients with certain neurological disorders to take a type of serotonin re-uptake inhibitor.