Histology Class

Digestive System

Light Microscopy Slide 27: With the unaided eye, note the general structural organization of the mucosa, epithelial lining, laminal propia, muscularis mucosa, submucosa, muscularis externa, and serosa. Identify the type of epithelium which lines the luminal surface of the esophagus. Observe the esophageal glands. Note the type of muscle in the muscularis externa and lower esophagus. Slide 28: Find places where Brunner’s glands connect with the crypts of lieberkuhn, Identify the two types of epithelial cells. Paneth cells. Slide 40: Nothing specified Slide 41: At low magnification observe: That the colon lacks intestinal villi, that the surface is studded with openings of straight crypts of lieberkuhn, the goblet cells, and that the solitary lymph follicles in the laminal propria often extend into the submucosa. Slide 71: With the unaided eye, note the general structural organization of the mucosa, epithelial lining, the laminal propria, sunmucosa, muscularis externa, and serosa. Identify the type of epithelium which lines the luminal surface of the esophagus, Distinguish between lamina prpria and muscularis mucosa. Note the typeof muscle in the muscularis externa and the lower esophagus. Slide 88: Use low magnification. Distinguish between gastric pits and gastric glands. Distinguish between chief cells and parietal cells. Observe the simple columnar surface mucous cells and that there are no goblet cells or brush border. Observe auerbach’s plexus. Some slides may show Meissner’s plexus. Note the size of the parietal cell and it’s characteristic pyramidal shape. Enteroendocrine cells. Note the capillaries and smooth muscle fibers in the lamina propria just subjacent to the surface mucous cell layer. Slide 93: Nothing specified Slide 192: Nothing specified Slide 201: With the unaided eye, note the general structural organization of the mucosa, epithelial lining, laminal propia, muscularis mucosa, submucosa, muscularis externa, and serosa. Identify the type of epithelium which lines the luminal surface of the esophagus. Observe the esophageal glands. Note the type of muscle in the muscularis externa and lower esophagus. Identify the endothelium of the central lacteal in the lamina propria. Locate paneth cells at the base of crypts. Try to distinguish them between argentaffin cells. Slide 202: Argentaffin cells. At low magnification observe: That the colon lacks intestinal villi, that the surface is studded with openings of straight crypts of lieberkuhn, the goblet cells, and that the solitary lymph follicles in the laminal propria often extend into the submucosa. Slide 204: No intestinal villi, has dense lymphocytes, and no argentaffin cells. At low magnification observe: That the colon lacks intestinal villi, that the surface is studded with openings of straight crypts of lieberkuhn, the goblet cells, and that the solitary lymph follicles in the laminal propria often extend into the submucosa. Slide 205: Identify the type of epithelium which lines the luminal surface of the esophagus. Observe the esophageal glands in the submucosa. Note the type of muscle in the muscularis externa and the middle and lower esophagus. Note the change in luminal epithelium from stratified squamous to simple columnar epithelium in the cardiac region of the stomach illustrating the cardio-esophageal junction. Slide 206: Observe the gastric pits of the pyloric stomach. Slide 207: Use low magnification. Distinguish between gastric pits and gastric glands. Distinguish between chief cells and parietal cells. Observe the simple columnar surface mucous cells and that there are no goblet cells or brush border. Observe auerbach’s plexus. Some slides may show Meissner’s plexus. Slide 208: Argentaffin cells and paneth cells. Slide 209: Argentaffin cells and paneth cells. Slide 213: Argentaffin cells Slide 264: Under 40X objective, analyze the distribution of the PAS-pos mucigen in the surface mucous cells of the gastric pits, mucous neck cells. Try to identify parietal cells and chief cells. Demo slide 827: Pyloric glands, observe the gastric pits of the pyloric stomach. Compare pyloric stomach with duodenum. Distinguish between gastric pits and intestinal intervillous space. Distinguish between gastric glands and crypts of lieberkahn. Find goblet cells and brush border. Note the organization of the lamina propria, muscularis mucosa, submucosa, and muscularis externa. Demo slide 969: Use low magnification. Distinguish between gastric pits and gastric glands. Distinguish between chief cells and parietal cells. Observe the simple columnar surface mucous cells and that there are no goblet cells or brush border. Observe Auerbach’s plexus. Some slides may show Meissner’s plexus. UT Demo slide 147: Compare pyloric stomach with duodenum. Distinguish between gastric pits and intestinal intervillous space. Distinguish between gastric glands and crypts of lieberkahn. Find goblet cells and brush border. Note the organization of the lamina propria, muscularis mucosa, submucosa, and muscularis externa. Also study argentaffin cells. UT Demo slide 153: Plasma cells, argentaffin cells. Electron Microscopy EM 3: Intestinal absorptive cells, goblet cells, brush border, mitochondrial distribution. EM 4: Intestinal absorptive cells EM 4b: Intestinal absorptive cells, brush border, cell junctions, and high density of mitochondria in the apical portion of these absorptive cells. EM 4c: Intestinal absorptive cells, note the intercellular spaces between absorptive cells in the epithelium lining. EM 14: Cells of the gastric gland, chief cells. EM 15: Cells of the gastric gland, distinguish between surface mucous cells and mucous neck cells. Identify mitochondria, and canaliculi. EM 16: Surface mucous, fundic stomach, distinguish between surface mucous cells and mucous neck cells. EM 17: Intestinal absorptive cells, macrophages, smooth muscle cells, fibroblasts, and endothelium. Note the intercellular space and its relationship to the basal lamina.

 

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