Image Analysis Lab - Texas A&M University

Research

Research

The Image Analysis Laboratory is set up as a core research facility in the College of Veterinary Medicine and Biomedical Sciences. Our goal is to provide the resources for research and training in the use of state-of-the art optical imaging technologies and high resolution electron microscopy. To meet the needs of the College and interdisciplinary faculties associated with the College, the Image Analysis Laboratory uses a three tiered facilities utilization strategy:

•  Independent research to develop and apply leading edge cellular imaging technologies.

•  Collaborative research to extend the applications base of faculty, staff, and students.

•  Routine fee for basic imaging services and applications training provided to faculty, staff, and students of the College and affiliated faculties and programs.

Research in the Image Analysis Laboratory encompasses the mission of the College of Veterinary Medicine and Biomedical Sciences emphasizing the microscopic structure of animal and human cells and the physiology of normal and diseased cells. Emphasis is placed on exploiting emerging technologies developed for non-invasive cellular imaging in addition to long-standing utilization of electron optical instruments for ultrastructural and cytochemical analyses.

Emerging technologies which employ fluorescent biosensors and biomarkers for evaluating the function of living cells and tissues are providing some of the most sensitive approaches for analysis of the molecular mechanisms of cellular function. Methods have been developed and/or adapted to exploit commercially available fluorescence probes and naturally occurring fluorescent molecules to quantitatively monitor numerous functional endpoints in cells.

Selected References

  1. Kim K, R Barhoumi, R Burghardt, S Safe (2005) Analysis of estrogen receptor a-SP1 interactions in breast cancer cells by fluorescence resonance energy transfer. Mol Endocrinol 19:843-854.

  2. Pietrantonio PV, C Jagge, S Taneja-Bageshwar, RJ Nachman, R Barhoumi (2005) The mosquito Aedes aegypti (L.) leucokinin receptor is a multiligand receptor for the three Aedes kinins. Insect Mol Biol 14:55-67.

  3. Joyce MM, FJ White, RC Burghardt, JJ Muniz, TE Spencer, FW Bazer, GA Johnson (2005) ISG15 conjugates to cytosolic proteins during the peri-implantation period and is expressed at the uterine-placental interface throughout pregnancy in sheep. Endocrinology 146:675-684.

  4. Wu Q, R Burghardt, S Safe (2004) Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERa) involves multiple domains of both proteins. J Biol Chem 279:53602-53612.

  5. Barhoumi R, J Faske, X Liu, RB Tjalkens (2004) Manganese potentiates lipopolysaccharide-induced expression of NOS2 in C6 glioma cells through mitochondrial-dependent activation of nuclear factor kappaB. Brain Res Mol Brain Res 122:167-179.

  6. Henderson CE, Hong MY, Barhoumi R, Burghardt RC, Carroll RJ, Turner ND, Chapkin RS, Lupton JR. (2004) Pro-oxidant environment of the colon compared to the small intestine may contribute to greater cancer susceptibility. Cancer Lett. 208(2):155-161.

  7. Fan YY, Ly LH, Barhoumi R, McMurray DN, Chapkin RS. Dietary docosahexaenoic acid suppresses T cell protein kinase C theta lipid raft recruitment and IL-2 production. (2004) J Immunol. 173(10):6151-6160.

  8. Spencer TE, GA Johnson, FW Bazer, RC Burghardt. (2004) Implantation Mechanisms: Insights from the sheep. Reproduction 128:657-668.

  9. Abdelrahim M, R Smith 3rd, R Burghardt, S Safe (2004) Role of Sp proteins in regulation of vascular endothelial growth factor expression and proliferation of pancreatic cancer cells. Cancer Res 64:6740-6749.

  10. Gray CA, DL Adelson, FW Bazer, RC Burghardt, EN Meeusen, TE Spencer (2004) Discovery and characterization of a novel epithelial-specific galectin in endometrium that forms crystals in trophectoderm. Proc Natl Acad Sci USA 101:7982-7987.

  11. Jiang J, D Dean, RC Burghardt , AR Parrish. Disruption of cadherin/catenin expression, localization and interactions during HgCl 2 -induced acute renal failure. Toxicol Sci, 80:170-182.

  12. Holmes SP, Barhoumi R, Nachman RJ, Pietrantonio PV. Functional analysis of a G protein-coupled receptor from the southern cattle tick Boophilus microplus (Acari: Ixodidae) identifies it as the first arthropod myokinin receptor. Insect Mol Biol. 2003 Feb;12(1):27-38.

  13. Meola S, H Sittertz-Bhatkar, P Langley, I Kasumba, S Aksoy (2003) Abdominal Pericardial Sinus: A neurohemal site in the tsetse and other cyclorraphan flies. J.Med. Entomol. 40:755-765.

  14. Johnson GA, RC Burghardt, FW Bazer, TE Spencer (2003) Osteopontin: Roles in implantation and placentation. Biol Reprod. 69:1458-1471.

  15. Cizmas L, R Barhoumi, R Burghardt, W Reeves, L He, T McDonald, KC Donnelly (2003) A comparison of two methods for fractionating complex mixtures in preparation for toxicity analysis. J Toxicol Environ Health A. 66:1351-1370.

  16. Johnson GA, RC Burghardt, MM Joyce, TE Spencer, FW Bazer, CA Gray, C Pfarrer (2003) Osteopontin is synthesized by uterine glands and a 45-kDa cleavage fragment is localized at the uterine-placental interface throughout ovine pregnancy. Biol Reprod 69:92-98.

  17. Johnson GA, RC Burghardt, TE Spencer, FW Bazer, MM Joyce, C Pfarrer, CA Gray (2003) Osteopontin expression in uterine stroma indicates a decidualization-like differentiation during ovine pregnancy. Biol Reprod 68:1951-1958.

  18. Burghardt RC, GA Johnson, LA Jaeger, H Ka, JE Garlow, TE Spencer, FW Bazer (2002) Integrins and extracellular matrix proteins at the maternal-fetal interface in domestic animals. Cells, Tissues, Organs 172:202-217.

  19. Meola S, H Sittertz-Bhatkar (2002) Neuroendocrine modulation of olfactory sensory neuron signal reception via axo-dendritic synapses in the antennae of the Mosquito, Aedes aegypti. J Mol Neuroscience 18: 239-245.

  20. Stoner M, B Saville, M Wormke, D Dean, R Burghardt, S Safe (2002) Hypoxia induces proteasome- dependent degradation of estrogen receptor alpha in ZR-75 breast cancer cells. Mol Endocrinol 16:2231-2242.

  21. Mealey KL, R Barhoumi, RC Burghardt, S Safe, DT Kochevar (2002) Doxycycline-induced expression of P-glycoprotein in MCF-7 breast carcinoma cells. Antimicrob Agents Chemother 46:755-761.

  22. Cossio-Bayguar R, R Barhoumi, RC Burghardt, GG Wagner, PJ Holman (2002) Basal cellular alterations of esterase, glutathione, glutathione S-transferase, intracellular calcium and membrane potentials in Coumaphos resistant Boophilus microplus (Acari: Ixodidae) cell lines. Pesticide Biochem Physiol 72:1-9.

  23. Gray CA, RC Burghardt, GA Johnson, FW Bazer, TE Spencer (2002) Evidence that an absence of endometrial gland secretions in uterine gland knockout ewes compromises conceptus survival and elongation. Reproduction,124:289-300.

  24. Barhoumi R, Y Mouneimne, I Awooda, SH Safe, KC Donnelly, RC Burghardt (2002) Characterization of calcium oscillations in normal and benzo[ a ]pyrene-treated Clone 9 cells. Tox Sci, 68: 444-450.

  25. Abdelrahim M, I Samudio, R Smith 3rd, R Burghardt, S Safe (2002) Small inhibitory RNA duplexes for Sp1 mRNA block basal and estrogen-induced gene expression and cell cycle progression in MCF-7 breast cancer cells. J Biol Chem, 277:28815-28822.

  26. Botos J, R Barhoumi, RC Burghardt, DT Kochevar (2002) Localization and kinetics of cell cycle-regulated RB phosphorylation in breast adenocarcinoma cells. In Vitro Cell & Develop Biol, 38:235-241.

  27. Reeves WR, R Barhoumi, R Burghardt, SL Lemke, K Mayura, TD Phillips, KC Donnelly (2001) Evaluation of methods for predicting the toxicity of polycyclic aromatic hydrocarbon mixtures. Environ Sci Technol 35:1630-1636.

  28. Johnson GA, FW Bazer, LA Jaeger, H Ka, JE Garlow, C Pfarrer, TE Spencer, RC Burghardt (2001) Muc-1, integrin and osteopontin expression during the implantation cascade in sheep. Biol Reprod 65:820-828.

  29. Barhoumi R, Y Mouneimne, KS Ramos, SH Safe, TD Phillips, VE Centonze, C Ainley, MS Gupta, RC Burghardt (2000) Analysis of benzo[ a ]pyrene partitioning and cellular homeostasis in a rat liver cell line. Toxicol Sci 53:264-270.

  30. Bowen JA, RC Burghardt (2000) Cellular mechanisms of implantation in domestic farm animals. Semin Cell Develop Biol 11:93-104.

    Other publications : Burghardt R link to PubMed; Rola Barhoumi link to PubMed