Skip to Main Content

Sequence-Based Microorganism Identification Services at the DNA Technologies Core Laboratory


* * Brand New Service * *

Sequence-based identification using the
MicroSeq Microbial Identification System (Applied Biosystems)

Please Note: We can only accept bacterial and fungal DNA at this time.
NO LIVE CULTURES

This project was supported by Texas A&M AgriLife, the Department of Veterinary Pathobiology in the College of Veterinary Medicine, and the Texas Veterinary Medical Diagnostic Laboratory.

SERVICES AND PRICING

  • 16S 500-bp Fragment Sequencing - $50.00
    • Primary method of identification
    • In most cases, 500-bp sequencing is sufficient to identify to species-level
    • Results within 24-hours of sample receipt
  • 16S Full Gene Sequencing - $100.00
    • Used to further resolve identity if 500-bp sequence yields ambiguous results
    • Can also be requested upfront (500-bp sequencing is not required to be performed first)
  • Fungal Sequencing - $50.00
    • Sequences D2 region of the large-subunit (LSU) rRNA gene
    • Results within 24-hours of sample receipt
  • PrepMan Ultra Sample Preparation Reagent (1 mL aliquot) - $15.00
    • Sufficient to extract DNA from 10 samples
    • Can be used on bacteria or fungi

FORMS AND INSTRUCTIONS

PROCESS

  • Check DNA quantity
    • NanoDrop ND-1000 Spectrophotometer
  • Amplify target region of gene
    • 16S universal primers or D2 LSU universal primers
  • Sequence PCR products
    • BigDye Terminator v1.1 chemistry
  • Analyze data using MicroSeq ID software and GenBank
    • Comparison against validated MicroSeq library and published GenBank submissions
  • Produce report containing results of data analysis