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Fragment Analysis Services
at the DNA Technologies Core Laboratory


Sample of microsatellite fragments (alleles) produced with fluorescent-tagged primers on
ABI 3130xl (6 multiplexed markers shown, dye set DS-31)

SERVICES AND PRICING

Client provides size standard
$1.50

Requestor provides PCR products and size standard

 
We provide size standard
$2.00

Requestor provides PCR products & we provide size standard

Mapmarker 400-ROX
96-well plate ready-to-run $100

Requestor provides 96-well plate of products in formamide ready to load with electronic sample sheet

Sample sheet template

(Instructions)

FORMS AND INSTRUCTIONS

SOFTWARE AND INSTRUMENTATION

ABI 3130xl Genetic Analyzer
  • All fragments are separated and detected on anABI 3130xl Genetic Analyzer
  • 96- or 384-well plate format
  • 16 samples per injection (run times vary from 20-40 minutes depending on desired read length)
  • 36-cm array with POP7 polymer capable of analyzing fragments up to 1000-bp
  • Sizing standards may be purchased through Applied Biosystems (GeneScan) or BioVentures (MapMarker)
  • Dye sets currently supported:

Dye Set
Filter Set
Size Standard Dye
Fragment Detection Dyes
DS-02
E5 (SNaPshot)
LIZ
dR110, dR6G, dTAMRA, dROX
DS-30
D
ROX
6-FAM, HEX, NED
DS-31
D
ROX
6-FAM, VIC, NED
DS-33
G5
LIZ
6-FAM, VIC, NED, PET

Analysis/Viewing Software
A NanoDrop ND-1000 Spectrophotomer
  • A NanoDrop ND-1000 Spectrophotomer is available for customer use to quantify DNA, primers, RNA, etc.

(view larger image)
ABI 3130xl Genetic Analyzer


(view larger image)
NanoDrop ND-1000 Spectrophotomer