Fragment Analysis Services
at the DNA Technologies Core Laboratory
Sample of microsatellite fragments (alleles) produced with fluorescent-tagged primers on
ABI 3130xl (6 multiplexed markers shown, dye set DS-31)
SERVICES AND PRICING
Client provides size standard |
$1.50 |
Requestor provides PCR products and size standard |
|
|---|---|---|---|
We provide size standard |
$2.00 |
Requestor provides PCR products & we provide size standard |
Mapmarker 400-ROX |
| 96-well plate ready-to-run | $100 | Requestor provides 96-well plate of products in formamide ready to load with electronic sample sheet |
FORMS AND INSTRUCTIONS
- Fragment analysis services order form (pdf)
- Instructions for submitting samples for fragment analysis (pdf)
- Sample sheet template (xls)
- Instructions to prepare sample sheet (pdf)
- Download results through FTP site
- View instructions for FTP site
SOFTWARE AND INSTRUMENTATION
ABI 3130xl Genetic Analyzer
- All fragments are separated and detected on anABI 3130xl Genetic Analyzer
- 96- or 384-well plate format
- 16 samples per injection (run times vary from 20-40 minutes depending on desired read length)
- 36-cm array with POP7 polymer capable of analyzing fragments up to 1000-bp
- Sizing standards may be purchased through Applied Biosystems (GeneScan) or BioVentures (MapMarker)
- Dye sets currently supported:
Dye Set |
Filter Set |
Size Standard Dye |
Fragment Detection Dyes |
|---|---|---|---|
DS-02 |
E5 (SNaPshot) |
LIZ |
dR110, dR6G, dTAMRA, dROX |
DS-30 |
D |
ROX |
6-FAM, HEX, NED |
DS-31 |
D |
ROX |
6-FAM, VIC, NED |
DS-33 |
G5 |
LIZ |
6-FAM, VIC, NED, PET |
Analysis/Viewing Software
- GeneMapper v3.7 is available for customer use on a first-come, first-served basis
- Peak Scanner v1.1 is available FREE through Applied Biosystems
A NanoDrop ND-1000 Spectrophotomer
- A NanoDrop ND-1000 Spectrophotomer is available for customer use to quantify DNA, primers, RNA, etc.
