Canine microsatellite multiplex set M07 (see below for legend)

Multiplexed Canine Microsatellites

Last updated 12.10.2002

Please note:  A correction is needed for the concentration of dNTPs in the multiplex paper.  In the paper we report that dNTP concentration is 0.5 mM for each dNTP in the final reaction.  The concentration should be 0.3 mM for each dNTP in the final reaction.  We apologize for any inconvenience.

To more efficiently collect genotype data for canine genomics projects we have developed multiplex sets for 155 canine microsatellites.

172 markers were originally termed Minimal Screening Set 1 (MSS1) and published as the first set of markers to use in canine linkage studies:  Richman M., Mellersh C.S., et al. (2001) Characterization of a minimal screening set of 172 microsatellite markers for genome-wide screens of the canine genome.  Journal of Biochemical and Biophysical Methods 47, 137-149.

We've multiplexed 155 of these markers into 48 sets of 2 to 5 markers, and the remaining 17 markers are amplified individually.  Amplification of the mutliplex sets is achieved using a single PCR mix and thermocycling program, varying only primer concentrations.

Multiplexed canine microsatellites reference:   Cargill EJ, Clark LA, Steiner JM, and KE Murphy (2002) Multiplexing of Canine Microsatellite Markers for Whole Genome Screens. Genomics 80(3):  250-253.

Click here for Cargill et al. (2002) [Table 1] showing multiplex sets corresponding to canine linkage groups and [Table 2] showing the multiplex sets.

A file of the multiplexed MSS1 markers sorted by canine linkage group and containing PIC values, heterozygosity values, marker type as well as references for each marker,   fluorescent dyes, number of observed alleles, observed product sizes, multiplex primer concentrations, multiplex sets, and verified primer sequences can be found in PDF format below.

Multiplexed Canine Microsatellite MSS1

Multiplexes are currently optimized for the ABI 310 Genetic Analyzer and ABI 377 Sequencer

Multiplex Set M07 Legend:

A:  tetra-repeat FH2096 labeled with 6FAM, allele sizes of 95 bp and 103 bp.

B:  tetra-repeat FH2548 labeled with HEX, allele sizes of 179 bp and 187 bp.

C:  tetra-repeat FH2079 labeled with TET, allele size of 275 bp.

D:  tetra-repeat FH2985 labeled with HEX, allele sizes of 310 bp and 318 bp.